• Axial focusing over several 100 micrometers within milliseconds
• Fast focusing/z-stacking
• No vibrations

Currently, most focusing solutions rely on mechanical axial movements, which can spoil the specimen. The optical focusing scheme of Optotune’s electrically tunable lenses (ETLs) allow for movement-free axial focusing within milliseconds. The EL-16-40-TC is ideally suitable for microscopy. Depending on the implementation and the optical performance requirements, focusing ranges of 60-1000 microns can be achieved.

Optotune's Laser Speckle Reducer offers a compact and effective solution to reduce laser speckle in high intensity illumination. By using a 4-f system, the LSR can be used in combination with multimode fibers.

Applications:

• Wide-field microscopy
• Confocal microscopy
• Two-photon fluorescence microscopy
• 3D light-sheet microscopy
• Transport-of-intensity (TIE) phase microscopy
• Quantitative phase contrast in digital holographic microscopy

Confocal microscopy
(pollen corn)

Two-photon microscopy
(stained neuronal cell  population)

Light-sheet microscopy
(zebra fish heart)

If magnification change is no issue it is recommended to mount the Optotune ETL directly after the microscopy objective, for example with the following adapter rings from Thorlabs:

• RMSA5 - Adapter with External C-Mount Threads and Internal RMS Threads
• RMSA6 - Adapter with External RMS Threads and Internal C-Mount Threads

Reference design package for a camera port adapter without mag change is available on request. Please contact This email address is being protected from spambots. You need JavaScript enabled to view it.

For speckle-free fluorescence illumination the following configuration is recommended:

More details can be found in the application note (see below).

Related products

EL-16-40-TC

EL-10-30-C

Laser Speckle Reducer